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ceramide synthesis  (Novus Biologicals)


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    Novus Biologicals ceramide synthesis
    Ceramide Synthesis, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 94/100, based on 29 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/ceramide+synthesis/Ceramide/pmc03948970-129-49-54
    Average 94 stars, based on 29 article reviews
    ceramide synthesis - by Bioz Stars, 2026-10
    94/100 stars

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    Article Title: Impact of insulin deprivation and treatment on sphingolipid distribution in different muscle subcellular compartments of streptozotocin-diabetic C57Bl/6 mice
    Article Snippet: .. Membranes were blocked with 5% fat-free milk before incubating overnight with primary rabbit anti-mouse antibodies for proteins of intracellular fatty acid uptake (CD36, ab64014; Abcam, Cambridge, MA), acyl-CoA synthesis (FATP1/ACSVL5, M-100; Santa Cruz Biotechnology, Dallas, TX), sphingolipid de novo synthesis [serine palmitoyltransferase (SPT) 10005260; Cayman Chemical, Ann Arbor, MI], ceramide synthesis (CerS1-NBP1–59733 and CerS5-NBP1–76964; Novus Biologicals, Littleton, CO), insulin sensitivity [Akt (Pan)] no. 2920, phospho-Akt (Ser 473 ) no. 4060, GSK-3α/β no. 5676, phospho-GSK-3β (Ser 9 ) no. 5558 (Cell Signaling Technology, Danvers, MA), and housekeeping protein (Vinculin, AB6039; Merck, Darmstadt, Germany). .. Proteins were detected using infrared fluorescent detection (Li-Cor Odyssey, Lincoln, NE) using appropriate anti-mouse and anti-rabbit secondary antibodies.



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    ERBB2-positive cells' sensitivity to palmitate is through ROS formation and ceramide production. (a) BT474 and MDA-MB-361 cells were treated with vehicle or 20 μmol/l GW9662 together with 0, 0.5, and 1 μmol/l of N -acetyl-cysteine (NAC) for 72 hours. Live cells were counted and presented as percentage of the GW9662 control. Error bars indicate the standard deviation from three individual experiments. *** P < 0.05. (b) BT474 and MDA-MB-361 cells were treated with vehicle or 20 μmol/l GW9662 together with 0 and 10 μmol/l of the ceramide synthesis inhibitor fumonisin B1 for 72 hours. Live cells were counted and presented as percentage of the GW9662 control. Error bars indicate the standard deviation from three individual experiments. ROS, reactive oxygen species.

    Journal: Breast Cancer Research : BCR

    Article Title: Peroxisome proliferator-activated receptor-γ protects ERBB2-positive breast cancer cells from palmitate toxicity

    doi: 10.1186/bcr2240

    Figure Lengend Snippet: ERBB2-positive cells' sensitivity to palmitate is through ROS formation and ceramide production. (a) BT474 and MDA-MB-361 cells were treated with vehicle or 20 μmol/l GW9662 together with 0, 0.5, and 1 μmol/l of N -acetyl-cysteine (NAC) for 72 hours. Live cells were counted and presented as percentage of the GW9662 control. Error bars indicate the standard deviation from three individual experiments. *** P < 0.05. (b) BT474 and MDA-MB-361 cells were treated with vehicle or 20 μmol/l GW9662 together with 0 and 10 μmol/l of the ceramide synthesis inhibitor fumonisin B1 for 72 hours. Live cells were counted and presented as percentage of the GW9662 control. Error bars indicate the standard deviation from three individual experiments. ROS, reactive oxygen species.

    Article Snippet: The PPARγ antagonist GW9662, the fatty acid palmitate, and the ceramide synthesis inhibitor fumonisin B1 were obtained from Sigma-Aldrich (St. Louis, MO, USA).

    Techniques: Standard Deviation